Adipic acid biosynthesis

Adipic acid biosynthesis Adipic acid is an important organic compound that can be obtained through biosynthesis. The biosynthesis of adipic acid is usually achieved through the use of metabolites produced during microbial fermentation processes.
A common method is to use microorganisms called Candida tropicalis for the biosynthesis of adipic acid. This microorganism has strong oxidation ability and substrate selectivity, and can convert fixed carbon sources into adipic acid.
The steps of biosynthesis of adipic acid generally include the following key reaction processes:
1. Substrate conversion: Select appropriate substrates, such as glucose, corn syrup, etc., and convert them into precursor substances of adipic acid through microbial metabolic pathways.
2. Biological oxidation: Utilizing the enzyme system of microorganisms to oxidize substrates and convert them into intermediate products of adipic acid. This step usually requires providing appropriate oxygen supply.
3. Separation and purification: Through appropriate separation and purification techniques, adipic acid in the fermentation broth is purified to remove impurities.
4. Post processing: Further processing of purified adipic acid, such as crystallization, drying, etc., to obtain the final adipic acid product.
It should be noted that in the biosynthesis process of adipic acid, the control of temperature, pH value, substrate concentration, oxygen supply and other conditions is very important. These conditions have a significant impact on the activity of microbial metabolism and product yield. Therefore, strict process control and optimization are required in practical operations.